dNTPs solutions
| dATP | dCTP | dGTP | dTTP | |
| Nomenclature | 2’-Deoxyadenosine 5’-triphosphate |
2’-Deoxycytidine 5’-triphosphate |
2’-Deoxyguanosine 5’-triphosphate |
2’-Deoxythymidine 5’-triphosphate |
| Chemical structure |
 |
 |
 |
 |
| CAS No. | 54680-12-5 | 109909-44-6 | 93919-41-6 | 27821-54-1 |
| Cation | Tri sodium salt | Tri sodium salt | Tri sodium salt | Tri sodium salt |
| Formula | C10H13N5Na3O12P3 | C9H13N3Na3O13P3 | C10H13N5Na3O13P3 | C10H14N2Na3O14P3 |
| Formula weight (g x mol-1) |
557.1 | 533.1 | 573.1 | 548.1 |
| Molar Extinction Coefficient [1] |
ε = 15.1 l x mmol-1 x cm-1; 259 nm |
ε = 8.9 l x mmol-1 x cm-1; 271 nm |
ε = 14.2 l x mmol-1 x cm-1; 252 nm |
ε = 9.5 l x mmol-1 x cm-1; 267 nm |
| Appearance | Clear colorless solution | Clear colorless solution | Clear colorless solution | Clear colorless solution |
Specification comparison
| Test | Commercial A | Commercial B | Commercial C | Pharmicell | |
| Nomenclature | Concentration | 100~110mM | 100±3mM | 100~110mM | 100~110mM |
| pH | 8.5±0.2(22℃) | 7.5 | 7.5~8.1 | 8.0~8.5 | |
| Stability | 24 month | 24 month | 42 month | 24 month | |
| Storage (℃) | -20 | -20 | -15~-25 | -20 | |
| HPLC | dNTP | ≥99.0% | ≥99.0% | ≥99.0% | ≥99.0% |
| dNDP | ≤0.5% | - | ≤0.9% | ≤0.5% | |
| Other impurities | ≤0.1% | - | - | ≤0.3% | |
| Functional | DNases, Rnases | Not detectable | Not detectable | Not detectable | Not detectable |
| Nicking activity | Not detectable | Not detectable | Not detectable | Not detectable | |
Optimal performance
RQ-PCR
Upon a comparative analysis of dNTPs in DNA and RNA amplification experiments,
the Ct values have been found to be withinthe range of ±1 when compared with other commercial dNTPs.
This observation unequivocally establishes that Pharmicell’s dNTPs have performance levels equivalent to
other commercial dNTPs
the Ct values have been found to be withinthe range of ±1 when compared with other commercial dNTPs.
This observation unequivocally establishes that Pharmicell’s dNTPs have performance levels equivalent to
other commercial dNTPs
Conventional PCR
The outcome of this test unequivocally verifies that Pharmicell's dNTPs meet the standards required for
commercial products.
It has also been confirmed that Pharmicell’s dNTPs do not contain any impurities that may affect
PCR amplification.
commercial products.
It has also been confirmed that Pharmicell’s dNTPs do not contain any impurities that may affect
PCR amplification.
